Chinese Journal OF Rice Science

Chinese Journal of Rice Science ›› 2012, Vol. 26 ›› Issue (5): 592-598.DOI: 10.3969/j.issn.10017216.2012.05.012

• Research Papers • Previous Articles     Next Articles

Identification of Pita and Pib Genes for Rice Blast Resistance of Rice Landraces from Yunnan Province

LI Jinbin 1, WANG Tian 2, XU Minghui 2,*   

  1. 1 Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences， Kunming 650205,China; 2 Key Laboratory of Biotechnology Research of Yunnan Province/ Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences/Key Laboratory of Southwest Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650223,China;
  • Received:2011-07-29 Revised:2012-05-22 Online:2012-09-10 Published:2012-09-10
  • Contact: XU Minghui2,*

云南地方稻种抗稻瘟病基因Pita和Pib的鉴定

李进斌1,王甜2,许明辉2,*   

  1. 1 云南省农业科学院 农业环境资源研究所， 云南 昆明 650205； 2 云南省农业科学院 生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创新重点实验室， 云南 昆明 650223；
  • 通讯作者: 许明辉2,*
  • 基金资助:

    国家自然科学基金资助项目（31160355）；云南省自然科学基金资助项目（2010ZC173）；云南省教育厅基金资助项目（09Y0198）。

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Abstract: The existence of the two genes of Pita and Pib in 176 rice landraces from Yunnan Province were rapidly determined using the dominant DNA markers of Pita/pita and Pib/pib alleles(YL155/YL18 and YL183/YL87 for the former, PibdomF/Pib dom R and Lys14SF/Lys145R for the latter designed according to their difference in sequence). The results showed that Pita  was found in 72 among 176 landraces with the frequency of  40.9%. Among them,  Pita  was found in 44 among 77 indica and 28 among 99 japonica cultivars, the frequency in indica and japonica were 57.1% and 28.3%, respectively. The distribution frequency of the resistance gene Pita in the japonica and indica varieties significantly differed, the frequency was higher in indica than in japonica cultivars. While Pib was found in 33 among 176 landraces with the frequency of  18.8%. Among them, Pib was found in 15 indica and 18 japonica cultivars, the frequency in indica and japonica were 19.5% and 18.2%, respectively.  Pita was mainly distributed in Yuxi, Baoshan, Lincang and Simao prefectures, while Pib was mainly distributed in Nujiang, Baoshan and Zhaotong prefectures of Yunnan Province. The results of pathogenic assays with the rice blast fungus in greenhouse were generally consistent with the results of molecular identification.

Key words: landrace, rice blast, molecular marker

摘要： 根据抗病等位基因Pita、Pib与pita、pib感病等位基因在DNA序列上的差异，合成Pita引物YL155/YL87和YL183/YL87，Pib基因引物PibdomF/PibdomR和Lys145F/Lys145R。利用这两套分子标记检测了176份云南地方稻种抗性基因Pita和Pib的分布。结果表明，供试材料中72份含有Pita基因，占40.9%，其中99份粳稻中有28份含有Pita基因，占28.3%；77份籼稻中有44份含有该基因，占57.1%；抗性基因Pita在粳稻和籼稻品种中的分布频率存在明显差异，籼稻高于粳稻；Pita基因主要分布于玉溪、保山、临沧、思茅等地区。而176份供试材料中仅有33份含有Pib基因，占18.8%，其中99份粳稻中有18份含有该基因，占18.2%；77份籼稻中有15份含有该基因，占19.5%；Pib基因主要分布在滇西部的怒江、保山和昭通等地区。分别选取具有代表性的18及17份材料在温室进行Pita和Pib基因抗性鉴定，结果与分子鉴定结果基本相符。

关键词: 地方稻种, 稻瘟病, 分子标记

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